Journal: Acta biomaterialia

Article Title: Spatial targeting of fibrosis-promoting macrophages with nanoscale metal-organic frameworks for idiopathic pulmonary fibrosis therapy.

doi: 10.1016/j.actbio.2023.12.006

Figure Lengend Snippet: Fig. 1. Overexpression of CSF-1R in CD206+ M2 macrophages. (A) Flow cytometry analysis of CSF-1R and CD206 expression in F4/80+ macrophages. Right panels: Quantified data in sorted F4/80+ macrophages. Results are expressed as means ± SD ( n = 3; ∗∗P < 0.01). (B) Representative results for coimmunostaining of CD206 and CSF-1R in the lung sections from bleomycin-treated mice. Representative images are shown. Bar = 20 μm. (C) Immunohistochemistry staining of CSF-1R and CD206 in the lung sections of IPF patients. Representative images are shown. Bar = 50 μm. (D-F) Single cell atlas of IPF patients according to dataset GSE122960. (D) Upper panels: Cellular populations identified. Lower panels: t-distributed stochastic neighbor embedding (t-SNE) depicting cell clusters originating either from a donor or from IPF patients. (E) Expression of CSF-1R for the cell types defined above each panel. (F) Percentage of cells with non-zero CSF-1R expression. (G) Kaplan– Meier survival analyses of IPF patients based on the expression of CSF-1R according to dataset GSE70866. (H) Immunofluorescence staining of CSF-1R and α-SMA on mouse lung tissues. Representative images are shown. Bar = 50 μm.

Article Snippet: The primary antibodies used were rabbit nti-phospho-CSF-1R, mouse anti- β-actin, and rabbit anti-GAPDH. horseradish peroxidase-conjugated goat anti-mouse/rabbit IgG Boster no. BA1050/BA1056, Wuhan, China, 1:10,0 0 0 dilution) was sed as the secondary antibody.

Techniques: Over Expression, Flow Cytometry, Expressing, Immunohistochemistry, Staining

Journal: Acta biomaterialia

Article Title: Spatial targeting of fibrosis-promoting macrophages with nanoscale metal-organic frameworks for idiopathic pulmonary fibrosis therapy.

doi: 10.1016/j.actbio.2023.12.006

Figure Lengend Snippet: Fig. 5. (A) Schematic diagram of the BLZ-loaded NPs therapy procedure. (B) Inhibitory effects of scrNP-BLZ@Mn-Cur or M2NP-BLZ@Mn-Cur on CSF-1R phosphorylation in lung tissues. (C) Representative flow cytometry results is shown to identify pulmonary macrophage subsets from lung digests in BLM-treated mice. Right panels: Quantified data in sorted F4/80+ macrophages ( n = 3, means ± SD). (D) Cell differential from BAL fluid was counted by Diff-Quik staining ( n = 5; mean ± SD; ∗p < 0.05). (E) The level of TGF- β1, IL-6, IL-11, and TNF- α in lung tissues were determined by ELISA. Results are expressed as means ± SD ( n = 3; ∗∗p < 0.01, ∗p < 0.05).

Article Snippet: The primary antibodies used were rabbit nti-phospho-CSF-1R, mouse anti- β-actin, and rabbit anti-GAPDH. horseradish peroxidase-conjugated goat anti-mouse/rabbit IgG Boster no. BA1050/BA1056, Wuhan, China, 1:10,0 0 0 dilution) was sed as the secondary antibody.

Techniques: Phospho-proteomics, Cytometry, Diff-Quik, Staining, Enzyme-linked Immunosorbent Assay

Journal: American journal of physiology. Heart and circulatory physiology

Article Title: miR-21-mediated decreased neutrophil apoptosis is a determinant of impaired coronary collateral growth in metabolic syndrome.

doi: 10.1152/ajpheart.00654.2014

Figure Lengend Snippet: Fig. 4. SD and JCR rats were treated with anti-miR-21 or scrambled anti-miR on day 5 RI or with anti-CD11b/CD18 and CD44 blocking antibodies on days 6–9 RI as indicated and euthanized on days 6 (12 h post-blocking antibody administration) or 9 RI, or untreated and euthanized on days 0, 3, 6, and 9 of RI. A–D: flow cytometry was performed on CZ and NZ perfusates using Gr-1 (Ly6G) antibodies to label neutrophils, CD115 antibodies to label monocytes, and annexin V antibodies to label apoptotic cells. Representative analysis (A and B) and cumulative data (C and D) are shown. *P 0.05 vs. sham; #P 0.05 vs. WKY RI; $P 0.05 vs. JCR RI. E and F: representative hematoxylin-and-eosin images ( 100) of remodeling collateral arteries (left) and large preexisting arteries (right) in cardiac cross sections from unperfused (E) and perfused (F) JCR rat hearts on day 9 RI. White arrows point to neutrophils. Note differences in size bars.

Article Snippet: Flow cytometry was performed on the elute using a Gr-1 antibody (MAb clone Ly6G, Acris, San Diego, CA) to label peripheral neutrophils, CD115 (MAb clone 2E11, Acris) antibody to label monocytes, CD3 (delta chain MAb clone 1F4, Arcis) antibody to label T cells, CD23 (IgE receptor, MAb clone 2G8, Arcis) to label mast cells, and annexin V (Life Technologies, Molecular Probes, Grand Island, NY) to label apoptotic cells.

Techniques: Blocking Assay, Cytometry

Journal: iScience

Article Title: Identification of potential biomarkers and therapeutic targets for antineutrophil cytoplasmic antibody-associated glomerulonephritis

doi: 10.1016/j.isci.2023.108157

Figure Lengend Snippet:

Article Snippet: The plasma proteins levels of CSF-1R (Boster, EK0807), C1Q (Invitrogen, BMS209), CSF-1 (Boster, EK0444), IL-34 (Boster, EK1365) and CRP (Boster, EK1316) were measured using the Quantikine enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Software